Modification of two capripoxvirus quantitative real-time PCR assays to improve diagnostic sensitivity and include beta-actin as an internal positive control
نویسندگان
چکیده
منابع مشابه
Validation of a genus-specific gene; TPS, used as internal control in quantitative Real Time PCR of transgenic cotton
Identification of genes with invariant levels of gene expression is a prerequisite for validating transcriptomic changes accompanying development. Ideally expression of these genes should be independent of the morphogenetic process or environmental condition.We report here the validation of internal control gene i.e.TPS (trehalose 6-phosphate-synthase) in cotton (Gossypium spp), using TaqMan sy...
متن کاملNovel internal controls for real-time PCR assays.
In the 19 years since the first descriptions of the PCR (1 ), nucleic acid amplification methods have made the transition from research to clinical laboratories. Molecular diagnostics are now firmly established as part of laboratory medicine, with applications in genetics, oncology, pharmacology, and infectious disease. Routine diagnostic applications of these methods have been made possible by...
متن کاملvalidation of a genus-specific gene; tps, used as internal control in quantitative real time pcr of transgenic cotton
identification of genes with invariant levels of gene expression is a prerequisite for validating transcriptomicchanges accompanying development. ideally expression of these genes should be independent of themorphogenetic process or environmental condition.we report here the validation of internal control genei.e.tps (trehalose 6-phosphate-synthase) in cotton (gossypium spp), using taqman syste...
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The multiplexing capabilities with different fluorescent dyes are limited in real-time PCR instruments equipped with one excitation source. Considering this limitation, a design was developed to create a triple-labeled probe as an internal positive control (IPC) that utilizes a combination of the fluorescence resonance energy transfer (FRET) and TaqMan techniques. The IPC probe, labeled with FA...
متن کاملReal-time multiplex PCR assays.
The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra. Spectral data are collected with discrete optics or dispersed onto an array for detect...
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ژورنال
عنوان ژورنال: Journal of Veterinary Diagnostic Investigation
سال: 2017
ISSN: 1040-6387,1943-4936
DOI: 10.1177/1040638717695609